ABSTRACT
Aims: Liver fibrosis is a chronic disease of the liver. This disease is a stage of passage to liver cancer. The objective of this work was to evaluate the ability of the ethanolic extract of Acanthospermum hispidumto block the progression of hepatic fibrosis induced in rats using diethylenitrosamine (DEN).Study Design:Study of the antifibrotic potential of extracts of Acanthospermum hispidum.Place and Duration of Study:In vivotests were performed from September 2018 to January 2019. The animal model tests were carried out in the pet shop of the Institute for Health Sciences Research (IRSS) of Burkina Faso and in the Cytogenetics Laboratory (FSS/ISBA) of the Republic of Benin.Methodology:The evaluationof the antifibrotic activity consisted in treating in wistarrats a liver fibrosis induced with the DEN which is a chemical agent whose effect on the liver has already been confirmed. As a result of the treatment, all animals were removed from the liver and blood. The livers were used for macroscopic and microscopic observations. Blood has been used for the evaluation of biochemical parameters in relation to fibrosis.Results:The analysis of the results of the biochemical parameters in relation to the fibrosis showed that the ethanolic extract of Acanthospermum hispidumat the dose of 250 mg / kg made it possible to obtain an improvement of these parameters compared to the other batches of animals. These results have been confirmed by those of the anatomo-pathological studies.Conclusion:The results of biochemical and histological analyzes revealed a capacity of Acanthospermum hispidumextracts to block the evolution of hepatic fibrosis in the rat. These results confirm the hepatoprotective potential of this medicinal plant used in traditional medicine in Burkina Faso
ABSTRACT
Pandiaka angustifolia (Vahl) Hepper, a species belonging to Amaranthaceae’s family is used in traditional medicine of Burkina Faso for the treatment of several diseases. The aim of this study was to evaluate the phenolics and flavonoids content, the antioxidant capacity, the lipoxygenase and xanthine oxidase inhibition potentials of Pandiaka angustifolia hydroacetonic macerate and fractions. Assay revealed that the Dichloromethane Fraction (DCMF) possess the best inhibitory activity of lipoxygenase, a key enzyme involved in inflammatory process with 83.84±1.89% of inhibition, as regards Xanthine Oxidase (XO) inhibiting percentage, n-hexane fraction (n-HF) exhibited the highest percentage of inhibition with 76.22 ± 4.78% at a concentration of 100μg/ml. Phenolics and flavonoids content in fractions and hydroacetonic macerate evaluation showed that hydroacetonic macerate possess the highest rate in total phenolic and the Dichloromethane fraction (DCMF) the highest rate in flavonoids content. This study showed that the apolar fractions of P. angustifolia is a potential natural source for the treatment of oxidative stress and inflammatory related diseases.
ABSTRACT
New and innovative antibacterial drugs research from plants is a best strategy to overcome the world problem about bacterial diseases. The aim of this study was to evaluate the in-vitro antibacterial activity and the phytochemical profile of various extracts from Hygrophila auriculata (Schumach.) Heine, Nelsonia canescens (Lam) Spreng and Peristrophe bicalyculata (Retz.) Nees; three acanthaceae species widely used in folk medicine of Burkina Faso. The inhibition diameter determination of the eleven bacteria strains and the Minimum Inhibition Concentration (MIC) of the plants extracts and fractions were evaluated using standards methods. All extracts were also evaluated for their phenolic contents. The results showed that all extracts and fractions exhibited good bacteria inhibition and have a wealth of phenolic compounds. Comparing the MIC of extracts and fractions, E. coli (CPI: 105182) strain was best inhibited and N. canescens butanol fraction presented the best activity. As regard phytochemical profile, highest values of total phenolics, total flavonoids, total flavonols and total tannins were obtained with N. canescens fractions. The results could justify the traditional used of these species against infectious diseases. This study shows that these three medicinal plants and especially N. canescens are good candidate for isolate new et innovative antibacterial compounds.